Heptaplex-direct PCR assay for simultaneous detection of foodborne pathogens
نویسندگان
چکیده
منابع مشابه
A novel magnetic capture–multiplex PCR assay for the simultaneous detection of three foodborne pathogens
doi: 10.1111/j.1757-837X.2011.00113.x Abstract Introduction Salmonella, Shigella and Escherichia coli O157: H7 are major foodborne pathogens that cause gastrointestinal diseases worldwide. Apart from food contamination, fecal pollution has been consistently associated with the transmission of these pathogens, and their rapid detection in food and stools is of significance for food safety. Howev...
متن کاملMolecular approaches for detection and identification of foodborne pathogens
Foodborne pathogens comprise microorganisms such as viruses, bacteria and parasites that can be transmitted by food and affect public health worldwide. The most common viruses transmitted via food are hepatitis A virus and Norwalk-like caliciviruses. Also, the most common bacteria involved in foodborne illnesses are Campylobacter jejuni, Clostridium perfringens, Salmonella spp, Escherichia...
متن کاملDevelopment and evaluation of a multiplex PCR for simultaneous detection of five foodborne pathogens.
AIMS To develop a rapid multiplex PCR method for simultaneous detection of five major foodborne pathogens (Staphylococcus aureus, Listeria monocytogenes, Escherichia coli O157:H7, Salmonella Enteritidis and Shigella flexneri, respectively). METHODS AND RESULTS Amplification by PCR was optimized to obtain high efficiency. Sensitivity and specificity assays were investigated by testing differen...
متن کاملDetection of Foodborne Pathogens
ments, food-producing companies, the agricultural sector, consumer organizations and four federal departments that share responsibility for food safety, i.e. Health Canada (HC), the Public Health Agency of Canada (PHAC), the Canadian Food Inspection Agency (CFIA), and Agriculture and Agri-Food Canada (AAFC) (Figure ). Part of the responsibility of the AAFC is to develop food-safety programs tha...
متن کاملMultiplex PCR assay for the simultaneous detection of four common food pathogens in meat
Accepted: 11/07/2014 Abstract The study was carried out to standardize a multiplex PCR assay for the simultaneous detection of four common food pathogens such as Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus and Salmonella enterica Typhimurium. Pathogen-specific DNA primers targeting hly A, ail, nuc and stm genes were employed for the identification of L. monocytogenes,...
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ژورنال
عنوان ژورنال: Forensic Science International: Genetics Supplement Series
سال: 2017
ISSN: 1875-1768
DOI: 10.1016/j.fsigss.2017.09.063